| Instrument Description
and Capabilities: The SPECTRAFLUOR
Plus is a fully automatic, computer controlled
Fluorescence, Absorbance and Luminescence instrument for measuring samples in a microplate. The instrument
fulfills the requirements for numerous fluorescence, luminescence and absorbance
applications in research and routine investigations.
- Fluorescence
- Time Resolved Fluorescence
TRF
- Fluorescence Intensity top
and bottom capabilities FI
- Fluorescence Resonanace Energy
Transfer FRET
- User choice of Fluorescence
filters included
- Absorbance reading mode (UV and VIS
range)
- User choice of Absorbance
filter included
- Temperature control and microplate-shaking included
- Adjustable bandwidth range
- Luminescence glow reading mode
- 6-1536-well plate capability
The filters are arranged in filter slides; four
excitation filters and four emission filters respectively can be
installed in the slides. Handles 6, 24, 96, 384
and 1536
well plates. Spectral range 360-700nm for fluorescence. 230-750nm
absorbance spectral range. Sensitivity ≤1.5 picogram fluorescein on each well or ≤4 fmol well. The
emission signal is measured by a highly sensitive PMT. Different
detection techniques with one instrument change of measurement
directions (top / bottom) by PC software. Thermal incubation at +5º C to +
40º C.
Dual optical channels allow measurements from above or below the plate
by mouse-click. Light to and from the samples is focused by a lens and
high quality filters are easily accessible
from the front of the instrument. Each filter slide can hold up to four
filters.
Orbital shaking and temperature control
are standard.
Suggested
use with black plates for bottom-to-top readings.
Optimal use with black plates prevent cross-contamination.
Specifications: |
| Modes of Operation |
|
| Light source |
High energy Xenon flash lamp |
| Detector |
Side window, low dark current
photomultiplier tube |
| Plate Shaking 3 intensities |
Linear and Orbital shaking with three different speeds |
| Fluorescence Spectral Filter Wavelength Range |
340 - 700 nm |
| Fluorescence Sensitivity 4FMOLE |
≤1.5pg fluorescein 200μL
≤4fmol/well in black plate |
Fluorescence Excitation Filters
(Choice of 2 filter pairs for Fluorescence readings) |
340 - 700 nm
Advise user's choice of filters |
Fluorescence Emission Filters
(Choice of 2 filter pairs for Fluorescence readings) |
340 - 700 nm
Advise user's choice of filters |
| Absorbance Spectral Filter Wavelength Range |
230-750nm |
| Absorbance Filters can be purchased |
Advise user's choice can be purchased |
| Absorbance Plate Types |
6, 24, 96, 384 and
1536
well plates |
| Luminescence Glow Type Wavelength Range |
400-700nm |
| Temperature range |
+5º C to + 42º C |
| Interface
connections |
RS 232
& parallel port |
Ordering information |
Catalog #P97081 Remanufactured |
Dimensions: LxWxH: 48 x 44 x 27 cm; weight 28 kg
Warranty
information:
Instruments come complete with NIST
certification and documentation, line cord and six days a week technical support. Remanufactured.
High quality instrument manufactured in Austria and
Germany and sold worldwide. Easily reset to 220 Volt.
Microplate Instrumentation
Sales, Service and Support since 1982.
Fluorescence Filters and Applications click here!
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