BMG FLUOstar Galaxy Multi-functional Microplate Reader

Measurement principles for the wide range of applications

The POLARstar is a fully automated microplate based multi-detection reader which incorporates numerous different measurement principles:

Fluorescence (UV and VIS range)
Time Resolved Fluorescence TRF
Fluorescence Intensity top to bottom capabilities FI
Fluorescence Resonanace Energy Transfer FRET
Polarization FP
User choice of Fluorescence filters included
Absorbance reading mode (UV and VIS range)
User choice of Absorbance filter included
Temperature control and microplate-shaking included
Adjustable bandwidth range
6-384-well plate capability

By integrating a high intensity xenon flash lamp and liquid light guide technology, the BMG models can cover the wavelength range from 240 to 740 nm in 96 well plate formats. Measurement Modes: Wavelength: Range 250nm to 740nm wavelength range, with the option to include up to 8 Excitation and 4 emission wavelengths with initial choice of 2 filter pairs. Measurement Time: 16 seconds for full 96 well plate reading.
Other Features can be included: Top-to-bottom reading, programmable shaking, 25 to 45 °C incubation and Polarization

Incubation

Temperature-regulated heating provide uniform incubation from 25 to 45°C.

Endpoint, slow and fast kinetic measurements

Kinetic applications with up to 250 kinetic points can be defined, with interval times from 100 ms to several hours. In addition, there is the possibility to measure where it is important in a kinetic study.

Multi-color detection

The fast filter wheels and the ability to define up to 8 sets of filters, each with individual gain and measurement timing parameters, allow analysis of multiple fluorophores in a single well (TRF, FRET, dual-emission, DNA-detection).

Specifications:

BMG FLUOstar Fluorescence and Absorbance Reader
BMG FLUORstar Galaxy Fluorescence and Absorbance Reader
BMG POLARstar Galaxy Fluorescence, Absorbance and Luminescence Reader

Measurement Principles	Fluorescence Standard Fluorescence Standard with FI, TRF, FRET, Polarization Absorbance
Fluorescence Wavelength Range	240-740nm spectral range
Fluorescence Filters	Two filter wheels can accomodate 8 filters and 4 filters each with your choice of 2 filter pairs installed in instrument
Absorbance Filters	One filter wheel can accomodate 4 filters each with your choice of 1 filter installed in instrument
Gain Control Adjustment (model upgraded)	Standard, FI, Time Resolved Fluorescence Mode, FRET, Polarization Absorbance Mode
Fluorescence Intensity (model upgraded)	Limit of detection: 0.9 fmol / well fluorescein ^{calculated according to the IUPAC standard: 3(SDblank)/slope}
Time-Resolved Fluorescence (model upgraded)	Limit of detection: 50 amol/well europium ^{calculated according to the IUPAC standard: 3(SDblank)/slope}
Absorbance	240-740nm spectral range Dynamic range: 0.000-3.000 OD Reproducibility: 0.030 OD for 2-3 OD range
Microplate Formats	96 well Fluorescence black plate and Absorbance clear plate
Reading Time	Dependent on test definition shortest reading time: 15 s for 96-well plate
Incubation	Incubation range from ambient +25°C to 45°C Temperature stability 0.2°C Temperature gradient < 0.5°C
Shaking	Orbital or linear shaking, user definable time and amplitude
Dimensions/weight	LxWxH: 48 x 44 x 27 cm; weight 28 kg
Interface connections	RS 232 serial & parallel port
Catalog # P21011	FLUORstar model Remanufactured $5,290.
Catalog # P21012	FLUORstar model upgraded Remanufactured
Catalog # P21004	FLUORstar Galaxy Remanufactured
Catalog # P21006	POLARstar Galaxy Remanufactured

Comes complete with line cord, NIST documentation and certification, 15 month warranty and six days a week technical support.

Microplate Instrumentation Sales, Service and Support since 1982.

Incubation Temperature-regulated heating provide uniform incubation from 25 to 45°C.

Incubation

Temperature-regulated heating provide uniform incubation from 25 to 45°C.