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Additional in-stock Multi-mode Detection
and Fluorescence Plate Readers:
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|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG FLUOstar|

|Biotek FL 600|Dynex Technologies MLX|

|ThermoFisher Finstruments Fluorescence|ThermoFisher Ascent|

|Tecan Spectrafluor|Tecan Spectrafluor Plus|Tecan Genios|

|Molecular Devices Gemini XS|ThermoFisher Luminoskan|

|ThermoFisher Luminoskan Ascent|

|BMG FLUOstar Galaxy|Perkin Elmer Victor 2 with or w/o injectors|


|BMG POLARstar Galaxy|Biotek FLX 800|Tecan Ultra|

|Molecular Devices Gemini EM|Tecan Safire|

Biotek Synergy 2 Fluorescence, Absorbance and
 Luminescence Reader SOLD

Bio-Tek Synergy 2

Instrument Description and Capabilities:

The Synergy 2 is BioTek's high-performance multi-mode plate reader designed for the life science research and drug discovery markets. Its fluorescence detection system uses deep blocking filters and dichroic mirrors for the best level of performance in research and screening applications. The dedicated absorbance detection system is monochromator-based, providing the best level of flexibility in this read mode. And, by utilizing dedicated optical elements for each individual detection technique, Synergy 2 offers uncompromised performance and the lowest limits of detection.

Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance. Its three broad-spectrum light sources have been chosen for optimal illumination and excitation in all applications.

The Synergy 2 is controlled by BioTek's Gen5™ Reader Control and Data Analysis Software, is certified for HTRF®, DLR™ and LanthaScreen™, and is compatible with AlphaScreen assays.

Detection method Fluorescence, Time Resolved Fluorescence, Fluorescence Polarization, Alpha Screen/ AlphaLisa, Luminescence, UV-Visible Absorbance
Read method End-point, kinetic, spectral scanning, well-area scanning
Microplate types 6- to 1536-well plates (luminescence 1-384)
PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control 4°C above ambient to 65°C
Shaking Included
PC Software Gen5™ to be purchased
Control through USB or serial port
Light source SQ Xenon Flash
Wavelength selection Monochromator
Wavelength range 200 - 999 nm, 1 nm increment
Bandpass 2.4 nm
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +/- 2 nm
Monochromator wavelength repeatability +/- 0.2 nm
OD accuracy < 1% at 2 OD typical
< 3% at 3.0 OD typical
OD linearity < 1% from 0 to 3.0 OD typical
OD repeatability < 0.5% at 2.0 OD typical
Stray light 0.03% at 230 nm typical
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Fluorescence Intensity
Sensitivity Fluorescein 1 pM typical (0.2 fmol/well 96-well plate; 0.1 fmol/well 384-well plate) - Top
Fluorescein 5 pM typical (1 fmol/well 96-well plate; 0.5 fmol/well 384-well plate) - Bottom
Light source Tungsten Halogen High Energy DPR Xenon Flash (optional)
Wavelength selection Deep blocking bandpass filters/dichroic mirrors
Wavelength range 300 - 700 nm with tungsten lamp (900 nm option)
200 - 700 nm with xenon lamp (900 nm option)
Dynamic range > 6 decades
Bandpass Filter dependent
Detection system PMT
Reading speed 96: 11 seconds
384: 22 seconds
1536: 42 seconds
Sensitivity 10 amol ATP typical (flash)
Wavelength range 300 - 700 nm
Dynamic range > 6 decades
Detection system Low noise PMT
Fluorescence Polarization
Light source Tungsten Halogen High Energy DPR Xenon Flash (optional)
Sensitivity 3 mP at 1 nM fluorescein typical
Wavelength selection Deep blocking filters/mirrors
Wavelength range 400 - 700 nm
Time-Resolved Fluorescence
Light source High Energy DPR Xenon Flash
Sensitivity Europium 60 fM typical (12 amol/well 96-well plate; 6 amol/well 384-well plate)
Wavelength selection Deep blocking filters/mirrors
Wavelength range 200 - 700 nM (900 nm option)
Reagent Dispensers
Number 2 syringe pumps (option)
Dispense volume 5 - 1000 µL in 1 µL increment
Dead volume 1.1 mL, 100 µL with backflush
Physical Characteristics
Dimensions 17"W x 17.5"D x 14.5"H (43.5 x 44.5 x 37.3 cm)
Weight 60 lbs (27 kg)
Catalog # P97238


Warranty information:

Instruments come complete with NIST certification and documentation, line cord and six days a week technical support. Remanufactured model with one year warranty.  High quality instrument made in USA. Easily reset to 220 Volt.

Microplate Instrumentation Sales, Service and Support since 1982.