Contact Us: 1.941.840.2591

Additional in-stock Multi-mode Detection
and Fluorescence Plate Readers:
Click on link to go to page

|Fluoroskan I|Perseptive Biosystems Cytofluor 4000|BMG FLUOstar|

|Biotek FL 600|Dynex Technologies MLX|

|ThermoFisher Finstruments Fluorescence|ThermoFisher Ascent|

|Tecan Spectrafluor|Tecan Spectrafluor Plus|Tecan Genios|

|Molecular Devices Gemini XS|ThermoFisher Luminoskan|

|ThermoFisher Luminoskan Ascent|

|BMG FLUOstar Galaxy|Perkin Elmer Victor 2 with or w/o injectors|


|BMG POLARstar Galaxy|Biotek FLX 800|BMG NOVOstar|Tecan Ultra|

|Tecan Genios Pro|Thermo Fisher Ascent FL with w/o injectors|

 |Molecular Devices M5|Molecular Devices Gemini EM|Tecan Safire|

ThermoFisher Scientific Fluoroskan Ascent FL
, TRF, FI, and Luminescence Microplate Reader
Equipped with UV-VIS Fluorescence and Luminescence Reader
 for top- and bottom- read assays $11,995.

All new filters, parts and 3 year warranty!


Instrument Description and Capabilities:

With two instruments in one, the Fluoroskan Ascent FL doubles your value.

Two-in-one fluorometric and luminometric reader for outstanding versatility and efficiency:

  • State-of-the-art optical system for unrivalled sensitivity
  • Highly-visual Ascent Software simplifies fluorometric and luminometric applications Ascent FL
  • On-board precision dispensers (various models) for rapid kinetic measurements on FL model only
  • Flexible plate formats, from 1 to 384 wells, enable reading in primary wells both models
  • Reads from above or below the plate both models
  • Precise temperature control provides optimum conditions for cell biology and enzyme assays both models
  • Variable speed orbital shaker for true mixing both models

Peak performance for fluorometric and luminometric measurements

The Fluoroskan Ascent and the two-in-one Fluoroskan Ascent FL set the standard in performance and ease of use. For even greater flexibility in the lab, the Fluoroskan Ascent FL is equipped with luminometric technology, complementing the long list of advanced features of the Fluoroskan Ascent, which is designed for fluorometric measurements. The Fluoroskan Ascent FL provides full coverage for fluorometric and glow and flash luminometric applications. Together with powerful  PC Software, it is a high-performance tool for research, industrial and clinical applications. 

The application areas of the Fluoroskan Ascent and Ascent FL models range from cell biology and immunology to molecular biology and microbiology. For many applications - including Ca2+ measurement, cell proliferation, cytotoxicity, hybridization assays, reporter gene assays, phagocytosis and immunoassays - both fluorometric and luminometric detection methods can be used.

Flexibility plus lower costs

The Fluoroskan Ascent FL doubles your value - reducing costs, improving efficiency and saving bench space in the lab. These benefits also apply to robot-controlled applications because just one system - hardware and software - covers both fluorometric and luminometric techniques. Even during a single session, the Fluoroskan Ascent FL provides the freedom to perform both types of measurements.

High-performance optics

The direct illumination optics of the Fluoroskan Ascent FL produces a highly focused light beam. In fluorescence measurement, this prevents crosstalk and ensures accurate readings. Two beam diameter settings, 3 mm and 1.5 mm, allow optimal readings of
1-96 and 384-well plates. Automatic gain selection eliminates the need for manual adjustment in fluorometry. It also guarantees the best possible sensitivity and dynamic range for all wavelengths. High sensitivity is one of the main benefits of fibreless optics -- and a critical feature for luminometry.

Market-leading dispenser system

For easy and accurate addition of reagents, up to three dispensers can be fitted on board the Fluoroskan Ascent and Ascent FL. Dispensing volumes can be adjusted in
5µl increments over a volume range of 5-1000µl.

Speed and precision are the key features of these dispensers. The system can dispense and measure simultaneously, which enables Ca2+ measurement and other rapid kinetic applications. The fast read time of the Fluoroskan Ascent FL, just 15 seconds for a 96-well plate, is another important advantage for kinetic applications.

On-board incubation and orbital shaking

An incubator option is available for cell biology, enzyme assays and other applications requiring precise temperature control.

Built-in orbital shaking, another advanced feature, speeds up reaction times and ensures effective mixing. Both the speed and amplitude of shaking can be adjusted.

Instruments come with 2 filter pairs of choice, line cord and three year warranty from Factory Trained Engineers.  Additional filter pairs can be purchased.

Technical Specifications:

Plate types: 1-, 6-, 12-, 24-, 48-, 96-, and 384-well plates
Can also be programmed for custom configurations.
Maximum dimensions 90 mm x 134 mm x 25 mm.
Optical Performance:

Light source: Quartz halogen lamp in stock
Detector: Photomultiplier tube
Excitation wavelength range: From 320 nm to 620 nm
Emission wavelength range: From 360 nm to 670 nm
Filters: High-quality interference filters. The excitation filter wheel can hold a maximum of eight filters, and the emission filter wheel can hold a maximum of six filters.
Excitation filters *)   standard 320 nm, 355 nm, 390 nm, 444 nm, 485 nm,
530 nm, 544 nm, 584 nm.
your custom filters available upon request.
Emissions filters *) standard 405 nm, 460 nm, 485 nm, 510 nm, 538 nm,
555 nm, 590 nm, 612 nm, 620 nm.
your custom filters available upon request.
*)Standard instrument includes 2 filter pairs of choice.
Performance Specifications

Fluoroskan Fluorometer
Theoretical sensitivity: 2 fmol fluorescein/well in a black 96-well plate
Dynamic Range: > 5 decades
Luminoskan Luminometer
Theoretical sensitivity: 5 fmol ATP / well in a white 96-well plate
(ATP Monitoring kit)
Dynamic range: 7 decades
Shaker: Orbital
Dispensers: 1 to 3 dispensers (optional)
Dispensing volume: 5-1000 µl in 5 µl increments
Accuracy: ±3 µl average
Precision: 5-15 µl < 5%, 20-1000 µl < 2%
Dispensing speed: 25 seconds per plate (96-well plate, 5µl/well)
Temperature range: From RT +3ºC to +45ºC (optional)
Interface Connections: RS 232 serial & parallel port

Catalog # P21024 Fluoroskan Ascent FL 
Catalog # P21026 Fluoroskan Ascent FL with one injector
Catalog # P21027 Fluoroskan Ascent FL with two injectors
Catalog # P21029 Fluoroskan Ascent FL with three injectors
Fluorescence Filters
and Applications

     click here!

Warranty information:

Instruments come complete with NIST certification and documentation, line cord and six days a week technical support. Remanufactured model with three year warranty.  High quality instrument made in Finland. Easily reset to 220 Volt.

Microplate Instrumentation Sales, Service and Support since 1982.